A1148,0025 25 ml A1148,0100 100 ml A1148,0250 250 TEMED（N,N,N',N'-四甲基乙二胺）_详细资料

产品信息

Synonym N,N,N',N'-Tetramethylethylenediamine, TMEDA

n 20°C/D 1.417

Boiling point 121°C

Formula C₆H₁₆N₂

M 116.21 g/mol

CAS-No. 110-18-9

HS-No. 29212900

EC-No. 203-744-6

Storage 2-8°C

LGK 3 A

Disposal 5

R 11-20/22-34

S 16-26-36/37/39-45

harmful, highly flammable, corrosive

Class/PG 3/II

UN-No. UN2372

WGK 1

Specification

Assay （GC） min. 99 %

Identity （IR） complies

Water （K.F.） max. 1 %

（1）  Needles, H.L. （1970） Anal. Biochem. 35, 533-537
Effect of solution components on large-pore polyacrylamide gel formation.
  （2）  Ogden, R.C. & Adams, D.A. （1987） Methods Enzymol. 152, 61-87
Electrophoresis in agarose and acrylamide gels.
  （3）  Gomes, A.V. & Barnes, J.A. （1998） Anal. Biochem. 260, 106-108
Gel electrophoresis of mini gels.

TEMED was introduced as an enhancer of the polymersitation （cross-linking） of acrylamide and bisacrylamide in gel electrophoresis. It catalysis the formation of free radicals of the initiator of the polymerisation, ammonium persulfate. If gels are degased to remove oxygen, add the TEMED after degasing. The working concentration is 50 μl / 100 ml of gel solution. TEMED is stable （< two years）, if it is protected from taking up water.
Caution: TEMED is harmful. Handle under a chemical fume hood. Do not breathe fumes!

Synonym	N,N,N',N'-Tetramethylethylenediamine, TMEDA
n 20°C/D	1.417
Boiling point	121°C
Formula	C₆H₁₆N₂
M	116.21 g/mol
CAS-No.	110-18-9
HS-No.	29212900
EC-No.	203-744-6
Storage	2-8°C
LGK	3 A
Disposal	5
R	11-20/22-34
S	16-26-36/37/39-45
	harmful, highly flammable, corrosive
Class/PG	3/II
UN-No.	UN2372
WGK	1
Specification
Assay （GC）	min. 99 %
Identity （IR）	complies
Water （K.F.）	max. 1 %

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