A5179,0025 Geno/miracle DNA Isolation Spin-Kit...

This kit is based on the unique anion exchange membrane with very high affinity to DNA. Cells or tissue are Iysed with a special Lysis Solution AC1.4 and Proteinase K. In the next step the Iysate, which contains DNA, is loaded onto a spin column. Negatively charged phosphates on the backbone of DNA interact with the positive charges on the surface of the membrane. Under medium salt conditions, impurities are washed off. Then, the pure DNA is eluted under high salt conditions, desalted and concentrated by an alcohol precipitation. To make the process of precipitation easier and to avoid an accidental loss of the pellet, a blue-coloured precipitation enhancer is added.