| Background: |
| | At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.
|
 | Description : |
| | Recombinant Human Interferon alpha-1b produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19392 Dalton.
The Interferon-alpha 1b gene was obtained from human leukocytes.
The IFN-alpha-1b is purified by proprietary chromatographic techniques.
|
| Physical Appearance: |
| | Sterile Filtered White lyophilized （freeze-dried） powder.
|
| Formulation: |
| | Lyophilized from a （1mg/ml） solution in containing 20mg HSA and 7.5mg sodium chloride. |
| Solubility: |
| | It is recommended to reconstitute the lyophilized IFN alpha-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
|
| Stability:
|
| | Lyophilized IFN-alpha-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IFN-alpha-1b should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.
|
| Purity: |
| | Greater than 98.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
|
| Amino acid sequence: |
| | The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Glu.
|
| Dimers and aggregates: |
| | Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
|
| Biological Activity: |
| | ProSpec's Interferon-alpha 1b is fully biologically active when compared to WHO standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 1 x 108 IU/ mg.
|
| Endotoxin: |
| | Less than 0.1 ng/µg （IEU/µg） of IFN-alpha 1b.
|
| Protein content: |
| | Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a standard solution of IFN-alpha 1b as a Reference Standard.
|
| Usage: |
| | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
沪公网安备 31011502008050号
