预装脱盐柱 HiTrap Desalting Column :快速脱盐缓冲液交换制备高低分子量分离。 球蛋白分离范围:1,000-5,000 柱体积:5ml
- Prepacked with Sephadex™ G-25 Superfine, the ready to use 5 ml HiTrap™ column is an excellent choice for fast, convenient desalting and buffer exchange using either a pump or a syringe.
- Replaces dialysis as the method of choice for desalting.
- Handles sample volume of 1.5 ml, or larger volumes by connecting up to five columns in series.
- Desalting requires less than one minute due to the high rigidity of Sephadex™ G-25 and the optimized packing technology.
- Connectors are included for easy connection to any chromatography system.
预装脱盐柱 HiTrap Desalting Column
HiTrap™ Desalting allows efficient, easy-to-perform group separations with a syringe or pump.
TECHNICAL SPECIFICATIONS |
HiTrap™ Desalting Column |
Column volume | 5 ml |
Medium | Sephadex™ G-25 Superfine |
Column dimensions | 1.6 × 2.5 cm |
Void volume | 1.5 ml |
Sample volume | 0.25-1.5 ml |
Elution volume | 1.0-2.0 ml |
Exclusion limit (Mr) | 5 × 103 globular protein |
Max. flow rate (H2O at 25°C) | 15 ml/min |
Recommended flow rate | 1-10 ml/min |
Max. pressure | 3 bar (43 psi, 0.3 MPa) |
Chemical stability | Stable in all commonly used buffer systems |
pH stability | 2-13 (long and short term) |
Storage | 20% ethanol |
Storage temperature | 4°C to 30°C |
Highly efficient desalting in 30 s using HiTrap™ Desalting Column.