G001 G418 disulfate/G418硫酸盐

G418 disulfate/G418硫酸盐
产品编号：G001 
产品规格：1g/5g/25g/100g

CAS Number	108321-42-2
Molecular Formula	C20H40N4O10 · 2 H2SO4
Molecular Weight	692.71 g/mol
Form	Powder
Purity Level	≥90.0%
Solubility	Water: Soluble
Source	Micromonospora Rhodorangea
Ammonia	<0.1%
Water Content (Karl Fisher)	<12.0%
Boiling Point	1012.1 °C
Flash Point	565.9 °C
Optical Rotation	+104o to +121o
Storage Conditions	2-8 °C

Description
G418 disulfate also known as geneticin, is an aminoglycoside antibiotic commonly used in plant and mammalian cell culture as a selectable marker.
TOKU-E offers three forms of G418: G418 disulfate (G001), G418 disulfate, EvoPure (G030), and G418 disulfate solution (G020). G418 disulfate and G418 disulfate, EvoPure? are soluble in aqueous solution. G418 disulfate solution is G418 dissolved in water at 50 mg/mL.  EvoPure? products are purified single antibiotic fractions, most >99% pure.
Mechanism
Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.
Plant Biology Applications
Gene selection (see below). Dong and McHughen (1992) compared the use of kanamycin and geneticin as a selection agent in the genetic transformation of flax by Agrobacterium mediated transformation. Results showed G418 to be the more potent selective agent. Experiments in sweet potato showed G418 to be a more reliable selection agent with a higher transformation efficiency compared to kanamycin.
Mammalian Biology Applications