Vimentin Mouse Monoclonal Antibody_详细资料

产品信息

货号： XY-0318 保存条件： Vimentin Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。抗体名： Vimentin Mouse Monoclonal Antibody 抗体英文名： Vimentin Mouse Monoclonal Antibody 保质期：一年抗原来源： Rabbit 克隆性：多克隆数量： 50 供应商：上海信裕生物科技有限公司规格： 50μl

Vimentin Mouse Monoclonal Antibody
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：

WB IP IF IHC ICC FC ELISA ChIP

1:1000 - 1:100 1:100 1:100 1:25-1:50 - -

抗体详细信息如下:

About this Antibody

Name Vimentin Mouse Monoclonal Antibody

Category Monoclonal antibody(mAb); Primary antibody

Isotype IgG1

Purification ProA affinity purified

About the Immunogen

Immunogen This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to a region of Vimentin.

Gene ID 7431

SwissProt P08670

Synonyms VIM

Category Cytoskeletal Signaling

Background Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. In essence, vimentin is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Vimentin has been shown to eliminate toxic proteins in JUNQ and IPOD inclusion bodies in asymmetric division of asymmetric division of mammalian cell lines. It has been used as a sarcoma tumor marker to identify mesenchyme. Methylation of the vimentin gene has been established as a biomarker of colon cancer and this is being utilized in the development of fecal tests for colon cancer. High levels of DNA methylation in the promotor region have also been associated with markedly decreased survival in hormone positive breast cancers.

包装清单：

产品编号产品名称包装

XY-0318 Vimentin Mouse Monoclonal Antibody 50μl

XY-050 Western一抗稀释液 50ml

— 说明书 1份

保存条件：
Vimentin Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品于专业人员的科学研究用，不得用于临床诊断或ZL，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
A. 按照推荐的稀释比例用信裕生物提供的Western一抗稀释液稀释抗体。
B. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
C. 回收稀释的一抗，4℃保存以备下次继续使用。
D. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。
2. 免疫染色：
可以使用信裕生物生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片：

Fig. 1. Western blot analysis of Vimentin on different cell lysates using anti- Vimentin antibody at 1/2000 dilution.Positive control:Lane 1: A549 Lane 2: Hela Lane 3: NIH/3T3 Lane 4: Mouse embryonic stem cell Fig. 2. Immunocytochemistry staining Vimentin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig. 3. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Vimentin antibody. Counter stained with hematoxylin. Fig. 4. Flow cytometric analysis of HeLa cells with Vimentin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).Goat anti mouse IgG (FITC) was used as the secondary antibody

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About this Antibody
Name	Vimentin Mouse Monoclonal Antibody
Category	Monoclonal antibody(mAb); Primary antibody
Isotype	IgG1
Purification	ProA affinity purified
About the Immunogen
Immunogen	This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to a region of Vimentin.
Gene ID	7431
SwissProt	P08670
Synonyms	VIM
Category	Cytoskeletal Signaling
Background	Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. In essence, vimentin is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Vimentin has been shown to eliminate toxic proteins in JUNQ and IPOD inclusion bodies in asymmetric division of asymmetric division of mammalian cell lines. It has been used as a sarcoma tumor marker to identify mesenchyme. Methylation of the vimentin gene has been established as a biomarker of colon cancer and this is being utilized in the development of fecal tests for colon cancer. High levels of DNA methylation in the promotor region have also been associated with markedly decreased survival in hormone positive breast cancers.

产品编号	产品名称	包装
XY-0318	Vimentin Mouse Monoclonal Antibody	50μl
XY-050	Western一抗稀释液	50ml
—	说明书	1份


Fig. 1. Western blot analysis of Vimentin on different cell lysates using anti- Vimentin antibody at 1/2000 dilution.Positive control:Lane 1: A549 Lane 2: Hela Lane 3: NIH/3T3 Lane 4: Mouse embryonic stem cell	Fig. 2. Immunocytochemistry staining Vimentin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig. 3. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Vimentin antibody. Counter stained with hematoxylin.	Fig. 4. Flow cytometric analysis of HeLa cells with Vimentin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).Goat anti mouse IgG (FITC) was used as the secondary antibody