【产品名称】:HE2302
小鼠白介素6(IL-6)elisa试剂盒
小鼠白介素6(IL-6)酶联免疫试剂盒
Mouse Interleukin 6,IL-6 ELISA KIT
实验原理:应用双抗体夹心法测定标本中小鼠白细胞介素6(IL-6)水平。用纯化的小鼠白细胞介素6抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入白细胞介素6,再与HRP标记的IL-6抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成终的黄色。颜色的深浅和样品中的IL-6呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中小鼠白细胞介素6(IL-6)浓度。
试剂盒组成:
试剂盒组成 |
48孔配置 |
96孔配置 |
保存 |
说明书 |
1份 |
1份 | |
封板膜 |
2片(48) |
2片(96) | |
密封袋 |
1个 |
1个 | |
酶标包被板 |
1×48 |
1×96 |
2-8℃保存 |
标准品:180ng/L |
0.5ml×1瓶 |
0.5ml×1瓶 |
2-8℃保存 |
标准品稀释液 |
1.5ml×1瓶 |
1.5ml×1瓶 |
2-8℃保存 |
酶标试剂 |
3 ml×1瓶 |
6 ml×1瓶 |
2-8℃保存 |
样品稀释液 |
3 ml×1瓶 |
6 ml×1瓶 |
2-8℃保存 |
显色剂A液 |
3 ml×1瓶 |
6 ml×1瓶 |
2-8℃保存 |
显色剂B液 |
3 ml×1瓶 |
6 ml×1瓶 |
2-8℃保存 |
终止液 |
3ml×1瓶 |
6ml×1瓶 |
2-8℃保存 |
浓缩洗涤液 |
(20ml×20倍)×1瓶 |
(20ml×30倍)×1瓶 |
2-8℃保存 |
【产品规格】:48T/96T
【试剂盒保存】:2-8℃
【有效期】:6个月
注:以上产品信息仅供科学研究实验使用,内容供参考,体产品信息请来电咨询。
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