About this protein |
Name | Recombinant Human Nesfatin-1 (Recombinant Human Nesfatin; rHuNesfatin-1);重组人Nesfatin-1蛋白 |
Synonyms | NEFA; NUCB2; Nucleobindin-2; DNA-binding protein NEFA; NEFAGastric cancer antigen Zg4; nucleobindin 2; nucleobindin-2; nucleobinding 2 |
Purity | >95% by SDS-PAGE and HPLC analyses. |
Biological Activity | Fully biologically active when compared to standard. The biological activity is tested by in vivo assay using healthy wild type male mice (C57BL/6J). |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4. |
Endotoxin | Less than 1EU/μg of rHuNesfatin-1 as determined by LAL method. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
Category | Others |
Background | Nesfatin is a metabolic polypeptide and is the N-terminal region of the precursor protein, Nucleobindin2 (encoded by NUCB2 gene). It is a naturally occurring protein and originally identified as a hypothalamic neuropeptide. Additionally, Nesfatin can be found in other areas of brain, and in pancreatic isletsβ-cells, gastric endocrine cells and adipocytes. It is responsible for regulating appetite and production of body fat. Excess nesfatin-1 in the brain leads to a loss of appetite, less frequent hunger, a "sense of fullness", and a drop in body fat and weight. A lack of nesfatin-1 in the brain leads to an increase of appetite, more frequent episodes of hunger, an increase of body fat and weight, and the inability to "feel full". |
Amino Acid Sequence | VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL |