About this Protein |
Name | Recombinant Cys-Protein A/G/L (Recombinant Cys-Protein A/G/L; Cys-Protein A/G/L);重组蛋白A/G/L,N端带Cys |
Synonyms | N/A |
Purity | >95% by SDS-PAGE and SEC-HPLC analyses. |
Biological Activity | The recombinant Protein A/G/L is a genetically engineered protein containing 5 IgG-binding regions of protein A, 2 of protein G and 5 of Protein L. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A/G/L to ensure the maximum specific IgG binding. The recombinant Protein A/G/L is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A/G/L binds to human, mouse, rat cow, goat, sheep, rabbit, guinea pig, pig, dog and cat IgG. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from additive free solution. |
Endotoxin | Less than 0.1EU/μg of Cys-Protein A/G/L as determined by LAL method. |
Reconstitution | Dissolve in distilled water or saline. |
Category | Protein A/G/L |
Background | Protein A/G/L is a genetically engineered protein that combines the IgG binding profiles of all Protein A, Protein G and Protein L. It is a gene fusion product. Recombinant fusion protein A/G/L contains five Ig-binding regions of protein L, five IgG binding domains from Protein A, and two Ig-binding region of protein G. Cell wall binding regions, albumin binding regions and other non-specific binding regions have all been eliminated from the fusion protein to ensure the maximum specific IgG binding. |
Amino Acid Sequence | CNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE GSGSGTYKLI LNGKTLKGET TTEAVDAATA EKVFKQYAND NGVDGEWTYD DATKTFTVTE KPEVIDASEL TPAVTTYKLV INGKTLKGET TTKAVDAETA EKAFKQYAND NGVDGVWTYD DATKTFTVTE EPRARPGSGS GKEETPETPE TDSEEEVTIK ANLIFANGST QTAEFKGTFE KATSEAYAYA DTLKKDNGEY TVDVADKGYT LNIKFAGKEK TPEEPKEEVT IKANLIYADG KTQTAEFKGT FEEATAEAYR YADALKKDNG EYTVDVADKG YTLNIKFAGK EKTPEEPKEE VTIKANLIYA DGKTQTAEFK GTFEEATAEA YRYADLLAKE NGKYTVDVAD KGYTLNIKFA GKEKTPEEPK EEVTIKANLI YADGKTQTAE FKGTFAEATA EAYRYADLLA KENGKYTADL EDGGYTINIR FAGKKVDEKP EEKEQVTIKE NIYFEDGTVQ TATFKGTFAE ATAEAYRYAD LLSKEHGKYT ADLEDGGYTI NIRFAG |