货号:
XGK1597
保存条件:
详见说明书
浓度:
1mg/1ml
应用范围:
WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
形态:
详见说明书
亚型:
IgG
免疫原:
KLH conjugated synthesised phosphopeptide derived from human GATA3 around the phosphorylation site of Ser308
适应物种:
详见说明书
宿主:
详见说明书
标记物:
详见说明书
抗体名:
磷酸化GATA结合蛋白3抗体
抗体英文名:
Anti-phospho-GATA3 (Ser308)
保质期:
详见说明书
抗原来源:
Rabbit
克隆性:
多克隆
目录编号:
详见说明书
级别:
详见说明书
靶点:
详见说明书
数量:
25
供应商:
上海西格
规格:
0.1ml/100μg 0.2ml/200μg
实验原理 :
(1)特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而,抗体可通过与病毒或毒素的特异性结合,直接发挥中和病毒的作用。
(2)活补体:IgM、IgG1、IgG2和IgG3可通过经典途径激活补体,凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
(3)结合细胞:不同类别的免疫球蛋白,可结合不同种的细胞,参与免疫应答。
(4)可通过胎盘及粘膜:免疫球蛋白G(IgG)能通过胎盘进入胎儿血流中,使胎儿形成自然被动
免疫。免疫球蛋白A(IgA)可通过消化道及呼吸道粘膜,是粘膜局部抗感染免疫的主要因素。
(5)具有抗原性:抗体分子是一种蛋白质,也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子,各具有不同的抗原性。
(6)抗体对理化因子的抵抗力与一般球蛋白相同:不耐热,60~70℃即被破坏。各种酶及能使蛋白质凝固变性的物质,均能破坏抗体的作用。抗体可被中性盐类沉淀。在生产上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白,再经透析法将其纯化。 产品名称 | 磷酸化GATA结合蛋白3抗体 |
英文名称 | Anti-phospho-GATA3 (Ser308)抗体 |
货号 | XGK1597 |
英文名称 Anti-phospho-GATA3 (Ser308)
中文名称 磷酸化GATA结合蛋白3抗体
别 名 GATA3 (phospho S308); p-GATA3 (phospho S308); GATA3 (phospho Ser308); p-GATA3 (Ser308); GATA 3; GATA3; GATA-3; GATA binding factor 3; GATA binding protein 3; HDR; MGC2346; MGC5199; MGC5445; Trans acting T cell specific transcription factor GATA 3; GATA3_HUMAN; Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3.浓 度 1mg/1ml规 格 0.1ml/100μg抗体来源 Rabbit克隆类型 polyclonal交叉反应 Human, Mouse, Dog, Cow, Rabbit, Sheep, Guinea Pig产品类型 一抗 磷酸化抗体研究领域 肿瘤 细胞生物 发育生物学 神经生物学 信号转导 转录调节因子 淋巴细胞 t-淋巴细胞蛋白分子量 predicted molecular weight: 49kDa性 状 Lyophilized or Liquid免 疫 原 KLH conjugated synthesised phosphopeptide derived from human GATA3 around the phosphorylation site of Ser308亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍 Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. Both GATA-4 and GATA-6 are found in heart, pancreas and ovary; lung and liver tissues exhibit GATA-6, but not GATA-4 expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites.Function : Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.Subcellular Location : Nucleus.Tissue Specificity : T-cells and endothelial cells.DISEASE : Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.Similarity : Contains 2 GATA-type zinc fingers.Database links : UniProtKB/Swiss-Prot: P23771.1抗体制备过程:
1.材料与试剂
a.提取的动物 Ig
b.弗氏完全佐剂和弗氏不完全佐剂
c.青霉素和链霉素
d.实验动物 兔
e.其它材料及试剂
2、选择实验活体。
3、进行动物免疫实验。
4、试取血样进行测试,查看免疫效果。
5、如果免疫成功,杀死实验活体,采集全部血清。
6、纯化出抗体。
7、鉴定抗体。胎牛血清(无菌采制)
我司提供以下技术外包服务:
1. Anti-phospho-GATA3 (Ser308)抗体分子生物学:质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学:基因合成、引物合成、基因测序、载体构建等
2.蛋白工程:原核、哺乳动物蛋白表达系统等
3.病毒包装:腺病毒、慢病毒等
4.抗体工程:磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
5.细胞工程:细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。
Anti-Alpha-Synuclein/FITC 荧光素标记核突触蛋白/突触素-1抗体IgGMulti-class antibodies规格: 0.2mlIDE, (Insulin degradation enzyme) 胰岛素降解酶(抗原)Multi-class antibodies规格: 0.5mg维受体-α 抗体 Anti-RAR-α 0.2mlSART3 英文名称: T淋巴细胞识别的鳞状细胞癌SART3抗体 0.2mlEF-CBP2 英文名称: 突触结合蛋白2抗体 0.2mlRhesus antibody Rh phospho-MK5(Ser142) 0酸化原活化蛋白激酶激酶5抗体 规格 0.1mlIDE, (Insulin degradation enzyme) 胰岛素降解酶(抗原)Multi-class antibodies规格: 0.5mgIntegrin Alpha 4/ITGA4(Integrin alpha 4) 整合素-α(抗原)Multi-class antibodies规格: 0.5mlAnti-CD24 CD24抗体Multi-class antibodies规格: 0.2mlRhesus antibody Rh PAK7/PAK5/MBT 激活激酶PAK7抗体 规格 0.2mlEFNA1 Kit Human 人 Ephrin-A1 / EFNA1 ELISA配对抗体 ELISATropomyosin 英文名称: 原肌球蛋白1抗体 0.1mlNLF3 英文名称: 限局性核因子3抗体 0.2mlAnti-CD24 CD24抗体Multi-class antibodies规格: 0.2ml5-HT/5 Hydroxy Tryptophan 英文名称: 5羟色胺抗体 0.1mlEME1 英文名称: 减数分裂内切酶EME1抗体 0.2ml合成酶-2抗体 Anti-NOS-2/iNOS 0.1mlAnti-Skp2/FITC 荧光素标记细胞S相激酶相关蛋白抗体IgGMulti-class antibodies规格: 0.2mlRhesus antibody Rh phospho-P53(Ser6) 0酸化YZ基因P53抗体 规格 0.1mlBDNF (Brain-derived Neurotrophin factor) 脑源神经营养因子(脑衍化神经营养因子)(多肽片断抗原)Multi-class antibodies规格: 0.5mg人肿瘤特异性抗原(TSA) ELISA 试剂盒 96T/48T 试剂盒 组装/原装Rat neuophil elastase (NE) ELISA Kit 大鼠中性粒细胞弹性蛋白酶(NE)ELISA试剂盒HumaissuePolypeptideAigen,TPAELISAKit 人组织多肽抗原(TPA)ELISA试剂盒 96T/48T 进口分装HumanHighdensitylipoprotein,HDLELISA试剂盒人高密度脂蛋白(HDL)ELISA试剂盒规格:96T/48T组织黑色素含量比色法定量检测试剂盒20次Humanlupusaicoagula,LAC/LAELISAKit人狼疮抗凝抗体(LAC/LA)ELISA试剂盒规格:96T/48TAnti-phospho-GATA3 (Ser308)抗体人α1抗胰糜蛋白酶(AACT)ELISA 试剂盒 96T/48T 试剂盒 组装/原装兔子β4整合素(ITG β4/CD104)免疫试剂盒 Rabbit iegrin β4,ITG β4/CD104 ELISA Kit热休克蛋白40(HSP-40)ELISA试剂盒 ,英文名: HSP-40 ELISA KitRabbit8-epi-prostaglandinF2alpha,8-epi-PGF2αELISA试剂盒兔8-异构前列腺素(8-epi-PGF2α)ELISA试剂盒规格:96T/48THumanAi-Compleme1qaibody,C1qELISA试剂盒人抗补体1q抗体(C1q)ELISA试剂盒规格:96T/48THumanBonemorphogeneticprotein2,BMP-2ELISAKit人骨成型蛋白2(BMP-2)ELISA试剂盒规格:96T/48T抗体的鉴定:
1)抗体的效价鉴定:鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2)抗体的特异性鉴定:抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争YZ试验测定。以不同浓度抗原和近似抗原分别做竞争YZ曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
3)真核翻译起始因子2C2抗体的亲和力:是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。