SB 203580 hydrochloride,869185-85-3_详细资料

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货号： abs812866 CAS号： 869185-85-3 供应商：爱必信(上海) 生物科技有限公司数量： 99 英文名： RWJ 64809 hydrochloride 保质期：见爱必信官网保存条件： store at -20℃ for one year（Powder）； in DMSO or others solvent store at 2-4℃ for two weeks, at -80℃ for six months. 规格： 10mg

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YZ剂描述：
产品名称：SB 203580 hydrochloride
产品别名：RWJ 64809 hydrochloride
英文别名：RWJ 64809 hydrochloride
靶点：p38 MAPK,PKB
CAS：869185-85-3
纯度：98%
外观：Powder
保存方法：store at -20℃ for one year（Powder）； in DMSO or others solvent store at 2-4℃ for two weeks, at -80℃ for six months.
描述：
SB 203580 hydrochloride is a p38 MAPK inhibitor with IC50 of 0.3-0.5 μM, also blocks PKB phosphorylation with IC50 of 3-5 μM.
溶解性：Soluble in ethanol (~30 mg/ml), DMSO (~30 mg/ml), DMF (~30 mg/ml), 1:10 solution of ethanol:PBS(pH 7.2) (~0.1 mg/ml), and water (25 mM).
体外研究：
SB 203580 (preincubated with 0-30 μM for 1 h and cultured for 24 h in the presence of 20 ng/mL IL-2) prevents the IL-2-induced proliferation of primary human T cells, murine CT6 T cells, or BAF F7 B cells with an IC₅₀ of 3-5 μM.
SB203580 blocks PKB phosphorylation (IC₅₀ 3-5 μM). SB203580 inhibitsthe phosphorylation of Ser473 in a dose-dependent manner in both CT6 and activated human T cells and IL-2-responsive BA/F3 F7 B cells.
Cell Proliferation Assay
Cell Line: CT6, BA/F3 cell line F7, and PBMC/T cells
Concentration: 0-30 μM
Incubation Time: Preincubated with 0-30 μM SB203580 for 1 h and cultured for 24 h in the presence of 20 ng/mL IL-2
Result: Prevented the IL-2-induced proliferation of primary human T cells, murine CT6 T cells, or BAF F7 B cells with an IC₅₀ of 3-5 μM.
体内研究：SB 203580 decreases protein concentrations of IL-1β from 106.49±10.93 to 67.85±7.39 pg/mL and TNF-α from 462.54±50.16 to 252.71±44.03 pg/mL. Similarly, the protein levels of MMP-2 and MMP-9 are significantly lower in the SB 203580 than the EM group. After treatment with SB 203580, the protein levels of MMP-2 and MMP-9 decreases from 2.70±0.14 to 1.74±0.26 ng/mL and from 3.17±0.31 to 1.98±0.24 ng/mL, respectively. SB 203580 is evaluated in several models of cytokine inhibition and inflammatory disease. It is demonstrated clearly to be a potent inhibitor of inflammatory cytokine production in both mice and rats with IC50 values of 15 to 25 mg/kg.
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