货号: K210007
相关应用:
DNA 纯化
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规格
High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
Sample Type (General): | Bacteria |
Downstream Application: | Cloning, In Vitro Transcription, Next-Generation Sequencing, Nucleic Acid Labeling, PCR, Sequencing, Transfection, Transformation |
Elution Volume - max: | 15 ml |
Elution Volume - min: | 15 ml |
Final Product: | BAC DNA, Plasmid DNA |
Isolation Technology: | Anion Exchange Resin |
Number of Reactions: | 25 Preps |
Plasmid Type: | <40kb, BAC, High Copy Plasmid, Low Copy Plasmid |
Product Line: | PureLink™ |
Product Size: | 25 preps |
Purification Time: | 2 hrs |
Purity or Quality Grade: | Transfection Grade |
Quantity: | 25 columns |
Sample Type (Specific): | Bacterial Culture |
System: | PureLink™ |
Yield (up to): | 1000 µg |
Shipping Condition: | Room Temperature |
内容及储存All components of the PureLink™ HiPure Plasmid DNA Purification Kits are shipped at room temperature. Upon receipt, store all components at room temperature.
Components:RNase A
(20 mg/ml in R3)
Lysis Buffer (L7)
Precipitation Buffer (N3)
Equilibration Buffer (EQ1)
Wash Buffer (W8)
Elution Buffer (E4)
TE Buffer (TE)
Columns描述快速简单的方案 - 不到 2 小时即可完成质粒 DNA 纯化
- 通过使用真空辅助的离心管,可节省特大量和超大量提取时间
PureLink™ HiPure 质粒纯化系统 (PureLink™ HiPure Plasmid Purification System) 设计用于以各种规模分离纯度的 (1) 质粒 DNA(表 1)。 简单的实验方案 - 阴离子交换树脂纯化的质粒 DNA(图 1)的质量相当于进行了两次氯化铯梯度离心,后者是Z严格的 DNA 纯化方法。 不到两个小时,DNA 的纯度就足以用于转染,无需通过额外步骤去除污染物,如 RNA、蛋白质和内毒素(图 2)。 另外,去除了酚、氯仿、溴化乙锭和氯化铯,因而程度避免了有害物质的接触和处理。
For Research Use Only. Not for use in diagnostic procedures.图表
Effect of DNA preparation on transfection.
温馨提示:不可用于临床ZL。
资料下载:
thermo-scientific-molecular-biology-workflow-solutions-brochure.pdf
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