Cytochrome c| Cytochrome c antibody

Product Name Cytochrome c antibody

Catalog No PAab098271

Packing 100ul

Form liquid

Alternative Name CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4

Purification Protein A+G purification

Purity 95% as determined by SDS-PAGE

Host Mouse

Isotype IgG2a

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.

Immunogen Cytochrome c

Specificity Human, Mouse, Rat

Tested Application ELISA, WB, IHC

Recommended dilution WB: 1:5000-1:30000; IHC: 1:100-1:1000

IHC use Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using PAab098271( Cytochrome c Antibody) at dilution of 1:600

WB use

Hela cells were subjected to SDS PAGE followed by western blot with PAab098271(Cytochrome c antibody) at dilution of 1:20000

Protein Information

Gene ID 54205

Uniprot ID P99999

Calculated MW 150 kDa

Research Area Cardiovascular, Metabolism