Asymmetric DiMethyl-Histone H3-R2 pAb_详细资料

产品信息

货号： PAB33585 保存条件： -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. 应用范围： WB,IHC,IF,IP,ChIP,ChIPseq 形态： Lyophilized or Liquid 亚型： IgG 适应物种： Human,Mouse,Rat,Other (Wide Range) 宿主： Rabbit 抗体名： Asymmetric DiMethyl-Histone H3-R2 pAb 抗体英文名： Asymmetric DiMethyl-Histone H3-R2 pAb 克隆性：多克隆靶点： Asymmetric DiMethyl-Histone H3-R2 规格： 10μl/20μl/50μl/100μl

Product Information：

Product name

Asymmetric DiMethyl-Histone H3-R2 pAb

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IHC,IF,IP,ChIP,ChIPseq

Species reactivity

Human,Mouse,Rat,Other (Wide Range)

Host Species

Rabbit

Purity

Affinity purification

Immunogen Information：

Immunogen

A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3

Calculated molecular weight

15kDa

Observed molecular weight

17kDa

Swiss Prot

Q16695

Gene ID (NCBI)

8290

Alternative Names

H3.4;H3/g;H3FT;H3t

Application Notes：

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IHC

1:50 - 1:200

IF

1:50 - 1:200

IP

1:50 - 1:200

ChIP

1:20 - 1:100

CHIPseq

1:20 - 1:100



Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R2 antibody.

Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R2 antibody.

Immunohistochemistry of paraffin-embedded rat brain using DiMethyl-Histone H3-R2 antibody.

Immunohistochemistry of paraffin-embedded human colon using DiMethyl-Histone H3-R2 antibody.

Immunohistochemistry of paraffin-embedded mouse brain using DiMethyl-Histone H3-R2 antibody.


Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R2 antibody.

Background：

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Storage：


Storage instructions

Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

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Asymmetric DiMethyl-Histone H3-R2 pAb

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Product name	Asymmetric DiMethyl-Histone H3-R2 pAb
Isotype	IgG
Form	Lyophilized or Liquid
Tested applications	WB,IHC,IF,IP,ChIP,ChIPseq
Species reactivity	Human,Mouse,Rat,Other (Wide Range)
Host Species	Rabbit
Purity	Affinity purification

Immunogen	A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3
Calculated molecular weight	15kDa
Observed molecular weight	17kDa
Swiss Prot	Q16695
Gene ID (NCBI)	8290
Alternative Names	H3.4;H3/g;H3FT;H3t

WB	1:500 - 1:2000
IHC	1:50 - 1:200
IF	1:50 - 1:200
IP	1:50 - 1:200
ChIP	1:20 - 1:100
CHIPseq	1:20 - 1:100