Recombinant Human Fibronectin/FN_详细资料

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货号： GOY-9637 是否是肿瘤细胞：否运输方式：免费运输年限：详见说明书生长状态：详见说明书数量： 42 供应商：上海谷研 ATCC Number： Recombinant Human Fibronectin/FN

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产品名称：Recombinant Human Fibronectin/FN
Known as   NovoNectin;Fibronectin; FN; Cold-insoluble globulin; CIG; FN; Fibronectin 1
Derived from   E.coli
Purity   Greater than 95% as determined by reducing SDS-PAGE.
Formulation   Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Shipping   The product is shipped at ambient temperature.
Storage   Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
Reconstitution   Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Endotoxin   Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Background   Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains,fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of ~63 kDa containing a central cell-binding domain, a high affinity heparin-binding domain II,and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors, and facilitates the gene transduction in the result.
Recombinant Human Fibronectin fragment is produced by our E.coli expression system and the target gene encoding Pro1270-Ser1546&Ala1721-Thr2016 is expressed.
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