美国BD采血管 单核细胞制备管 PRP管 CPT管 362760 36276 362753BD Vacutainer® CPT™ Mononuclear Cell Preparation Tube - Sodium Citrate

BD Vacutainer® CPT™ Mononuclear Cell Preparation Tube - Sodium Citrate

PREPARATION AND STORAGE

Store BD Vacutainer® CPT™ Tubes upright at room temperature (18-25° C). Protect tubes from direct light. Shelf life at 18-25ºC is one year from the date of manufacture.

 

Description

 

BD Vacutainer® CPT™ is a fully-closed system for separation of mononuclear cells from whole blood, where cell separation is carried out in the primary blood collection tube. This decreases the complexity of steps for mononuclear cell separation, thereby minimizing variability from sample processing.

CPT™ is an evacuated, sterile blood collection tube containing buffered sodium citrate or sodium heparin anticoagulant, liquid density medium and an inert gel barrier. It is intended for the collection of whole blood and the subsequent separation of mononuclear blood cells for the purposes of in vitro diagnostic examination.

Whole blood is drawn directly into the CPT™ using standard phlebotomy techniques and processed in the same tube. During centrifugation, the gel forms a physical barrier between the mononuclear cells in plasma and the erythrocytes and granulocytes. The separated, concentrated suspension of mononuclear cells in plasma can then be transported in the primary blood collection tube.

使用BD CPT™管，应在室温（18-25ºC）。

2.用BD一次性静脉采血针将血液采进入采血管。

3.收集后，将BD  CPT管应被轻轻地180度上下颠倒混匀，持续8-10次
在室温下垂直储存管，直到离心。两小时内采血的血液样本离心效果Z好。
离心条件：相对离心力1500g-1800g,15-20分钟，25℃室温

4.在室温下（18-25ºC），离心管/血液样本需在1500到1800的RCF（相对离心力）下离心至少20分钟。
注：离心之前，CPT管应被轻轻地180度上下颠倒混匀，持续8-10次。另外，要检查该管是放在适当的离心机中，注意要对称放置管子，以双数例如2支，4支对称放置在离心机中。

警告：过度的离心速度（超过2000 RCF）可能导致断管和血液暴露以及其他可能的损害。对于一个给定的RCF要计算正确的离心速度，使用下面的公式：
转速设定值=(（RCF）x100000)/(（1.12）X（r）)
其中r（以厘米）是从离心管底到离心ZX柱的径向距离，CPT管处在水平位置，RCF是相对离心力，在这种情况下,为1500–1800g。
5．离心后，PRP位于Z上层（血浆层）的底部（见图）。不干扰细胞层的情况下，可用注射器或吸管之类的抽吸出PRP部分并用于注射。

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