JM110 感受态细胞(化转)/ JM110 chemically E.coli Express Competent Cells_详细资料

产品信息

货号： CHC00043 CAS号：无供应商： LSM Bio 数量：货源充足英文名： JM110 chemically E.coli Express Competent Cells 保质期： 6month 保存条件：负80摄氏度规格： 0.1ml*10

JM110 Chemically Competent Cells
JM110 Competent Cells Components

JM110: 100μl/tube

pUC19 (control vector锛?0pg/μl): 10μl

Storage at -80鈩?for 6month

JM110 Chemically Competent Cells Genotype rpsL (Strr) thr leu thi-1 lacY galK galT ara tonA tsx dam dcm supE44 Δ(lac-proAB) /F′ [traD36 proAB lacIqlacZΔM15] JM110 Chemically Competent Cell Description JM110 strains with streptomycin sulfate resistance (Strr); methylation gene dam, DCM deletion strain, plasmid DNA extracted, can be dam, DCM methylation sensitive endonuclease cutting; lacIqlacZ M15 JM110 can make a blue white selection, but the conversion efficiency is not high, generally not used for plasmid construction that is only suitable for plasmid transformation. JM110 receptive cells were made by special process, and the conversion efficiency of pUC19 plasmid was about 107 cfu/ mu g DNA JM110 Chemically Competent Cell Transformation Protocol 1. Please store JM110 Chemically Competent Cells in -80°C for late use. Take it then insert the tube in the ice immediately for 5 minutes. 2. Add your plasmid DNA (vector) to the cell mixture. Carefully flick the tube 4–5 times to mix cells and DNA. Do not vortex.Place the mixture on ice for 25 minutes,Do not mix. 3. Place it in the 42°C water for 45 seconds,then immediately transfer the tube back into ice for 2min. Do not mix. 4. Add 700ul LB or 2YT liquid nutrient medium (No antibiotic). then put on the Constant temperature shaker (200 rpm) for 1 hours at 37°C. 5.Pellet the mixture by centrifugation at 5000rpm/min for 1min. Pour off about 100ul of cell supernatant, then resuspend the cell pellet in the remaining medium by gently vortexing the tube. Spread the cell on the LB or 2YT Resistance plates by antibiotic, then inverted and Incubate overnight at 37°C.(bule white selection should place the plate at 37°C for 14 hours or more).
温馨提示：不可用于临床ZL。

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