alpha 1 microglobulin ELISA KIT|Human

Catalog  EF001337

Packing 48T/96T

Reactivity Human

Detect Range 0.625-40ng/ml

alpha 1 microglobulin ELISA KIT Sensitivity< 0.375ng/ml

Application alpha 1 microglobulin ELISA KIT|Human  is for detecting the concentration of Human alpha 1 microglobulin in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade

Principle of alpha 1 microglobulin ELISA KIT|Human

The microtiter plate provided in alpha 1 microglobulin ELISA KIT|Human has been pre-coated with an Human alpha 1 microglobulin antibody specific to Human alpha 1 microglobulin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human alpha 1 microglobulinantibody preparation specific for Human alpha 1 microglobulin then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human alpha 1 microglobulin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human alpha 1 microglobulinin the samples is then determined by comparing the O.D. of the samples to the standard curve.

*Please noted that alpha 1 microglobulin ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!