Human Methionyl- tRNA synthetase, cytoplasmic, MARS ELISA KIT

Product Name:Human Methionyl- tRNA synthetase, cytoplasmic, MARS ELISA KIT
Packing:96T

Catalog No.:ELI-53117h

Gene Name:MARS

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Methionyl-tRNA synthetase(cytoplasmic(MARS

Alternative Name:MARS,Methionyl-tRNA synthetase(cytoplasmic(MARS
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Methionyl- tRNA synthetase, cytoplasmic, MARS ELISA KIT allows for the in vitro quantitative determination of Methionyl-tRNA synthetase(cytoplasmic(MARS concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Methionyl- tRNA synthetase, cytoplasmic, MARS ELISA KIT has been pre-coated with an Methionyl-tRNA synthetase(cytoplasmic(MARS antibody specific to Methionyl-tRNA synthetase(cytoplasmic(MARS .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Methionyl-tRNA synthetase(cytoplasmic(MARS and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Methionyl-tRNA synthetase(cytoplasmic(MARS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Methionyl-tRNA synthetase(cytoplasmic(MARS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!