Human Colorectal mutant cancer protein, MCC ELISA KIT Product Name:Human Colorectal mutant cancer protein, MCC ELISA KIT
Packing:96T
Catalog No.:ELI-50594h
Gene Name:MCC
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Colorectal mutant cancer protein(MCC
Alternative Name:MCC,Colorectal mutant cancer protein(MCC
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Colorectal mutant cancer protein, MCC ELISA KIT allows for the in vitro quantitative determination of Colorectal mutant cancer protein(MCC concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Colorectal mutant cancer protein, MCC ELISA KIT has been pre-coated with an Colorectal mutant cancer protein(MCC antibody specific to Colorectal mutant cancer protein(MCC .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Colorectal mutant cancer protein(MCC and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Colorectal mutant cancer protein(MCC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Colorectal mutant cancer protein(MCC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!