Human CD48 antigen, CD48 ELISA KIT Product Name:Human CD48 antigen, CD48 ELISA KIT
Packing:96T
Catalog No.:ELI-50290h
Gene Name:CD48
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:CD48 antigen(CD48
Alternative Name:CD48,CD48 antigen(CD48
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human CD48 antigen, CD48 ELISA KIT allows for the in vitro quantitative determination of CD48 antigen(CD48 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human CD48 antigen, CD48 ELISA KIT has been pre-coated with an CD48 antigen(CD48 antibody specific to CD48 antigen(CD48 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CD48 antigen(CD48 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CD48 antigen(CD48, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of CD48 antigen(CD48 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!