Human Gamma- glutamyl hydrolase, GGH ELISA KIT

Product Name:Human Gamma- glutamyl hydrolase, GGH ELISA KIT
Packing:96T

Catalog No.:ELI-47204h

Gene Name:GGH

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Gamma-glutamyl hydrolase(GGH

Alternative Name:GGH,Gamma-glutamyl hydrolase(GGH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Gamma- glutamyl hydrolase, GGH ELISA KIT allows for the in vitro quantitative determination of Gamma-glutamyl hydrolase(GGH concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Gamma- glutamyl hydrolase, GGH ELISA KIT has been pre-coated with an Gamma-glutamyl hydrolase(GGH antibody specific to Gamma-glutamyl hydrolase(GGH .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Gamma-glutamyl hydrolase(GGH and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Gamma-glutamyl hydrolase(GGH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Gamma-glutamyl hydrolase(GGH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!