Human Dehydrodolichyl diphosphate synthase, DHDDS ELISA KIT

Product Name:Human Dehydrodolichyl diphosphate synthase, DHDDS ELISA KIT
Packing:96T

Catalog No.:ELI-46932h

Gene Name:DHDDS

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Dehydrodolichyl diphosphate synthase(DHDDS

Alternative Name:DHDDS,Dehydrodolichyl diphosphate synthase(DHDDS
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Dehydrodolichyl diphosphate synthase, DHDDS ELISA KIT allows for the in vitro quantitative determination of Dehydrodolichyl diphosphate synthase(DHDDS concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Dehydrodolichyl diphosphate synthase, DHDDS ELISA KIT has been pre-coated with an Dehydrodolichyl diphosphate synthase(DHDDS antibody specific to Dehydrodolichyl diphosphate synthase(DHDDS .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Dehydrodolichyl diphosphate synthase(DHDDS and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Dehydrodolichyl diphosphate synthase(DHDDS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Dehydrodolichyl diphosphate synthase(DHDDS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!