Human Deleted in malignant brain tumors 1 protein, DMBT1 ELISA K

Human Deleted in malignant brain tumors 1 protein, DMBT1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

DMBT1,GP340; muclin; SAG; deleted in malignant brain tumors 1 protein; glycoprotein 340; gp-340; hensin; salivary agglutinin; surfactant pulmonary-associated D-binding protein; deleted in malignant brain tumors 1

Search name

Human DMBT1 ELISA KIT ,Human GP340 ELISA KIT ,Human muclin ELISA KIT ,Human SAG ELISA KIT ,Human deleted in malignant brain tumors 1 protein ELISA KIT ,Human glycoprotein 340 ELISA KIT ,Human gp-340 ELISA KIT ,Human hensin ELISA KIT ,Human salivary agglutinin ELISA KIT ,Human surfactant pulmonary-associated D-binding protein ELISA KIT ,Human deleted in malignant brain tumors 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Deleted in malignant brain tumors 1 protein, DMBT1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to DMBT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DMBT1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DMBT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DMBT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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