Human Dihydrolipoyl dehydrogenase mitochondrial, DLD ELISA KIT

Human Dihydrolipoyl dehydrogenase mitochondrial, Dld ELISA KIT

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Dihydrolipoyl dehydrogenase mitochondrial,Dihydrolipoamide dehydrogenase, Dld,GCSL,LAD,PHE3,Dihydrolipoamide dehydrogenase,Glycine cleavage system L protein,DLDD,DLDH,GCSL,LAD,PHE3,E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex,diaphorase,dihydrolipoyl dehydrogenase, mitochondrial,glycine cleavage system L protein,glycine cleavage system protein L,lipoamide dehydrogenase,lipoamide reductase,lipoyl dehydrogenase

Search name

Human Dihydrolipoyl dehydrogenase mitochondrial ELISA KIT ,Human Dihydrolipoamide dehydrogenase ELISA KIT ,Human Dld ELISA KIT ,Human GCSL ELISA KIT ,Human LAD ELISA KIT ,Human PHE3 ELISA KIT ,Human Dihydrolipoamide dehydrogenase ELISA KIT ,Human Glycine cleavage system L protein ELISA KIT ,Human DLDD ELISA KIT ,Human DLDH ELISA KIT ,Human GCSL ELISA KIT ,Human LAD ELISA KIT ,Human PHE3 ELISA KIT ,Human E3 component of pyruvate dehydrogenase complex ELISA KIT ,Human 2-oxo-glutarate complex ELISA KIT ,Human branched chain keto acid dehydrogenase complex ELISA KIT ,Human diaphorase ELISA KIT ,Human dihydrolipoyl dehydrogenase ELISA KIT ,Human mitochondrial ELISA KIT ,Human glycine cleavage system L protein ELISA KIT ,Human glycine cleavage system protein L ELISA KIT ,Human lipoamide dehydrogenase ELISA KIT ,Human lipoamide reductase ELISA KIT ,Human lipoyl dehydrogenase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human dihydrolipoyl dehydrogenase, mitochondrial,DLDH concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to DLDH, During the reaction, DLDH in the sample or standard competes with a fixed amount of biotin-labeled DLDH for sites on a pre-coated Monoclonal antibody specific to DLDH. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DLDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.