Human Melanocortin receptor 5, MC5R ELISA KIT

Product Name:Human Melanocortin receptor 5, MC5R ELISA KIT
Packing:96T

Catalog No.:ELI-45983h

Gene Name:MC5R

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Melanocortin receptor 5(MC5R

Alternative Name:MC5R,Melanocortin receptor 5(MC5R
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Melanocortin receptor 5, MC5R ELISA KIT allows for the in vitro quantitative determination of Melanocortin receptor 5(MC5R concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Melanocortin receptor 5, MC5R ELISA KIT has been pre-coated with an Melanocortin receptor 5(MC5R antibody specific to Melanocortin receptor 5(MC5R .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Melanocortin receptor 5(MC5R and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Melanocortin receptor 5(MC5R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Melanocortin receptor 5(MC5R in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!