Human Transcriptional- regulating factor 1, TRERF1 ELISA KIT Product Name:Human Transcriptional- regulating factor 1, TRERF1 ELISA KIT
Packing:96T
Catalog No.:ELI-45739h
Gene Name:TRERF1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Transcriptional-regulating factor 1(TRERF1
Alternative Name:TRERF1,Transcriptional-regulating factor 1(TRERF1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Transcriptional- regulating factor 1, TRERF1 ELISA KIT allows for the in vitro quantitative determination of Transcriptional-regulating factor 1(TRERF1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Transcriptional- regulating factor 1, TRERF1 ELISA KIT has been pre-coated with an Transcriptional-regulating factor 1(TRERF1 antibody specific to Transcriptional-regulating factor 1(TRERF1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Transcriptional-regulating factor 1(TRERF1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Transcriptional-regulating factor 1(TRERF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Transcriptional-regulating factor 1(TRERF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!