Human Qprt ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Human Qprt ELISA KIT ,Human quinolinate phosphoribosyltransferase ELISA KIT ,Human HEL-S-90n ELISA KIT ,Human QPRTase ELISA KIT ,Human nicotinate-nucleotide pyrophosphorylase [carboxylating] ELISA KIT ,Human epididymis secretory sperm binding protein Li 90n ELISA KIT ,Human nicotinate-nucleotide pyrophosphorylase (carboxylating) ELISA KIT
Intended use
Human Qprt ELISA kit allows for the in vitro quantitative determination of Human Qprt concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Human Qprt ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Qprt and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Qprt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Qprt in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Qprt ELISA kit Range 0.15-10ng/ml
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