Human Glutaryl- CoA dehydrogenase, mitochondrial, GCDH ELISA KIT

Human Glutaryl-CoA dehydrogenase mitochondrial, GCDH ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

GCDH,GCD; ACAD5; glutaryl-Coenzyme A dehydrogenase; glutaryl-CoA dehydrogenase,Glutaryl-CoA dehydrogenase mitochondrial

Search name

Human GCDH ELISA KIT ,Human GCD ELISA KIT ,Human ACAD5 ELISA KIT ,Human glutaryl-Coenzyme A dehydrogenase ELISA KIT ,Human glutaryl-CoA dehydrogenase ELISA KIT ,Human Glutaryl-CoA dehydrogenase mitochondrial ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human GCDH concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Human Glutaryl-CoA dehydrogenase mitochondrial, GCDH ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GCDH, During the reaction,GCDHin the sample or standard competes with a fixed amount of biotin-labeled GCDH for sites on a pre-coated Monoclonal antibody specific to GCDH. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GCDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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