Human Glutaminyl- tRNA synthetase, QARS ELISA KIT Product Name:Human Glutaminyl- tRNA synthetase, QARS ELISA KIT
Packing:96T
Catalog No.:ELI-41067h
Gene Name:QARS
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Glutaminyl-tRNA synthetase(QARS
Alternative Name:QARS,Glutaminyl-tRNA synthetase(QARS
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Glutaminyl- tRNA synthetase, QARS ELISA KIT allows for the in vitro quantitative determination of Glutaminyl-tRNA synthetase(QARS concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Glutaminyl- tRNA synthetase, QARS ELISA KIT has been pre-coated with an Glutaminyl-tRNA synthetase(QARS antibody specific to Glutaminyl-tRNA synthetase(QARS .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Glutaminyl-tRNA synthetase(QARS and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glutaminyl-tRNA synthetase(QARS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Glutaminyl-tRNA synthetase(QARS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!