Human Vasoactive intestinal polypeptide receptor 2, VIPR2 ELISA

Human Vasoactive intestinal polypeptide receptor 2, VIPR2 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

VIPR2,VPAC2; C16DUPq36.3; DUP7q36.3; PACAP-R-3; PACAP-R3; VIP-R-2; VPAC2R; VPCAP2R; PACAP type III receptor; VIP and PACAP receptor 2; helodermin-preferring VIP receptor; pituitary adenylate cyclase-activating polypeptide type III receptor; vasoactive intestinal polypeptide receptor 2; vasoactive intestinal peptide receptor 2

Search name

Human VIPR2 ELISA KIT ,Human VPAC2 ELISA KIT ,Human C16DUPq36.3 ELISA KIT ,Human DUP7q36.3 ELISA KIT ,Human PACAP-R-3 ELISA KIT ,Human PACAP-R3 ELISA KIT ,Human VIP-R-2 ELISA KIT ,Human VPAC2R ELISA KIT ,Human VPCAP2R ELISA KIT ,Human PACAP type III receptor ELISA KIT ,Human VIP and PACAP receptor 2 ELISA KIT ,Human helodermin-preferring VIP receptor ELISA KIT ,Human pituitary adenylate cyclase-activating polypeptide type III receptor ELISA KIT ,Human vasoactive intestinal polypeptide receptor 2 ELISA KIT ,Human vasoactive intestinal peptide receptor 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Vasoactive intestinal polypeptide receptor 2, VIP-R-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to VIP-R-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for VIP-R-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain VIP-R-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VIP-R-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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