Human Pachytene checkpoint protein 2 homolog, TRIP13 ELISA KIT Product Name:Human Pachytene checkpoint protein 2 homolog, TRIP13 ELISA KIT
Packing:96T
Catalog No.:ELI-35638h
Gene Name:TRIP13
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Pachytene checkpoint protein 2 homolog(TRIP13
Alternative Name:TRIP13,Pachytene checkpoint protein 2 homolog(TRIP13
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Pachytene checkpoint protein 2 homolog, TRIP13 ELISA KIT allows for the in vitro quantitative determination of Pachytene checkpoint protein 2 homolog(TRIP13 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Pachytene checkpoint protein 2 homolog, TRIP13 ELISA KIT has been pre-coated with an Pachytene checkpoint protein 2 homolog(TRIP13 antibody specific to Pachytene checkpoint protein 2 homolog(TRIP13 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Pachytene checkpoint protein 2 homolog(TRIP13 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pachytene checkpoint protein 2 homolog(TRIP13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Pachytene checkpoint protein 2 homolog(TRIP13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!