Human Activating molecule in BECN1- regulated autophagy protein

Human Activating molecule in BECN1- regulated autophagy protein 1, AMBRA1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Ambra1,DCAF3; WDR94; DDB1 and CUL4 associated factor 3; WD repeat domain 94; activating molecule in BECN1-regulated autophagy protein 1; activating molecule in beclin-1-regulated autophagy; autophagy/beclin-1 regulator 1

Search name

Human Ambra1 ELISA KIT ,Human DCAF3 ELISA KIT ,Human WDR94 ELISA KIT ,Human DDB1 and CUL4 associated factor 3 ELISA KIT ,Human WD repeat domain 94 ELISA KIT ,Human activating molecule in BECN1-regulated autophagy protein 1 ELISA KIT ,Human activating molecule in beclin-1-regulated autophagy ELISA KIT ,Human autophagy/beclin-1 regulator 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Activating molecule in BECN1- regulated autophagy protein 1, AMBRA1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to AMBRA1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AMBRA1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AMBRA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AMBRA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.