Human Acyloxyacyl hydrolase, AOAH ELISA KIT

Product Name:Human Acyloxyacyl hydrolase, AOAH ELISA KIT
Packing:96T

Catalog No.:ELI-34429h

Gene Name:AOAH

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Acyloxyacyl hydrolase(AOAH

Alternative Name:AOAH,Acyloxyacyl hydrolase(AOAH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Acyloxyacyl hydrolase, AOAH ELISA KIT allows for the in vitro quantitative determination of Acyloxyacyl hydrolase(AOAH concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Acyloxyacyl hydrolase, AOAH ELISA KIT has been pre-coated with an Acyloxyacyl hydrolase(AOAH antibody specific to Acyloxyacyl hydrolase(AOAH .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Acyloxyacyl hydrolase(AOAH and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Acyloxyacyl hydrolase(AOAH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Acyloxyacyl hydrolase(AOAH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!