Human ATP synthase subunit delta, mitochondrial, ATP5D ELISA KIT

Product Name:Human ATP synthase subunit delta, mitochondrial, ATP5D ELISA KIT
Packing:96T

Catalog No.:ELI-34156h

Gene Name:ATP5D

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:ATP synthase subunit delta(mitochondrial(ATP5D

Alternative Name:ATP5D,ATP synthase subunit delta(mitochondrial(ATP5D
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human ATP synthase subunit delta, mitochondrial, ATP5D ELISA KIT allows for the in vitro quantitative determination of ATP synthase subunit delta(mitochondrial(ATP5D concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human ATP synthase subunit delta, mitochondrial, ATP5D ELISA KIT has been pre-coated with an ATP synthase subunit delta(mitochondrial(ATP5D antibody specific to ATP synthase subunit delta(mitochondrial(ATP5D .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ATP synthase subunit delta(mitochondrial(ATP5D and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ATP synthase subunit delta(mitochondrial(ATP5D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of ATP synthase subunit delta(mitochondrial(ATP5D in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!