Human DNA2- like helicase, DNA2 ELISA KIT Product Name:Human DNA2- like helicase, DNA2 ELISA KIT
Packing:96T
Catalog No.:ELI-31725h
Gene Name:DNA2
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:DNA2-like helicase(DNA2
Alternative Name:DNA2,DNA2-like helicase(DNA2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human DNA2- like helicase, DNA2 ELISA KIT allows for the in vitro quantitative determination of DNA2-like helicase(DNA2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human DNA2- like helicase, DNA2 ELISA KIT has been pre-coated with an DNA2-like helicase(DNA2 antibody specific to DNA2-like helicase(DNA2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for DNA2-like helicase(DNA2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DNA2-like helicase(DNA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of DNA2-like helicase(DNA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!