Human Melanoma- associated antigen B16, MAGEB16 ELISA KIT

Product Name:Human Melanoma- associated antigen B16, MAGEB16 ELISA KIT
Packing:96T

Catalog No.:ELI-31500h

Gene Name:MAGEB16

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Melanoma-associated antigen B16(MAGEB16

Alternative Name:MAGEB16,Melanoma-associated antigen B16(MAGEB16
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Melanoma- associated antigen B16, MAGEB16 ELISA KIT allows for the in vitro quantitative determination of Melanoma-associated antigen B16(MAGEB16 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Melanoma- associated antigen B16, MAGEB16 ELISA KIT has been pre-coated with an Melanoma-associated antigen B16(MAGEB16 antibody specific to Melanoma-associated antigen B16(MAGEB16 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Melanoma-associated antigen B16(MAGEB16 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Melanoma-associated antigen B16(MAGEB16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Melanoma-associated antigen B16(MAGEB16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!