Human Cartilage matrix protein, MATN1 ELISA KIT Product Name:Human Cartilage matrix protein, MATN1 ELISA KIT
Packing:96T
Catalog No.:ELI-31477h
Gene Name:MATN1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Cartilage matrix protein(MATN1
Alternative Name:MATN1,Cartilage matrix protein(MATN1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cartilage matrix protein, MATN1 ELISA KIT allows for the in vitro quantitative determination of Cartilage matrix protein(MATN1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cartilage matrix protein, MATN1 ELISA KIT has been pre-coated with an Cartilage matrix protein(MATN1 antibody specific to Cartilage matrix protein(MATN1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cartilage matrix protein(MATN1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cartilage matrix protein(MATN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cartilage matrix protein(MATN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!