Human KDM1A (Lysine- specific histone demethylase 1A) ELISA Kit

Human KDM1A (Lysine- specific histone demethylase 1A) ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Intended use

Human KDM1A ELISA kit allows for the in vitro quantitative determination of Human KDM1A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

Human KDM1A ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for KDM1A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain KDM1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of KDM1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.31-20ng/ml

Human KDM1A ELISA kit manual download

Order Human KDM1A ELISA kit by email:sales@lifescience-market.com