Human Spindle and kinetochore- associated protein 2, SKA2 ELISA

Human Spindle and kinetochore- associated protein 2, SKA2 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

SKA2,FAM33A; family with sequence similarity 33, member A; spindle and KT (kinetochore) associated 2; spindle and kinetochore-associated protein 2; spindle and kinetochore associated complex subunit 2

Search name

Human SKA2 ELISA KIT ,Human FAM33A ELISA KIT ,Human family with sequence similarity 33 member A ELISA KIT ,Human spindle and KT (kinetochore) associated 2 ELISA KIT ,Human spindle and kinetochore-associated protein 2 ELISA KIT ,Human spindle and kinetochore associated complex subunit 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Spindle and kinetochore- associated protein 2, SKA2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to SKA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SKA2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SKA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SKA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.