Human Scaffold attachment factor B1, SAFB ELISA KIT

Product Name:Human Scaffold attachment factor B1, SAFB ELISA KIT
Packing:96T

Catalog No.:ELI-29739h

Gene Name:SAFB

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Scaffold attachment factor B1(SAFB

Alternative Name:SAFB,Scaffold attachment factor B1(SAFB
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Scaffold attachment factor B1, SAFB ELISA KIT allows for the in vitro quantitative determination of Scaffold attachment factor B1(SAFB concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Scaffold attachment factor B1, SAFB ELISA KIT has been pre-coated with an Scaffold attachment factor B1(SAFB antibody specific to Scaffold attachment factor B1(SAFB .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Scaffold attachment factor B1(SAFB and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Scaffold attachment factor B1(SAFB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Scaffold attachment factor B1(SAFB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!