Human Ganglioside GM2 activator, GM2A ELISA KIT

Human Ganglioside GM2 activator, GM2A ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Gm2a,GM2-AP; SAP-3; cerebroside sulfate activator protein; ganglioside GM2 activator; shingolipid activator protein 3; sphingolipid activator protein 3; GM2 ganglioside activator

Search name

Human Gm2a ELISA KIT ,Human GM2-AP ELISA KIT ,Human SAP-3 ELISA KIT ,Human cerebroside sulfate activator protein ELISA KIT ,Human ganglioside GM2 activator ELISA KIT ,Human shingolipid activator protein 3 ELISA KIT ,Human sphingolipid activator protein 3 ELISA KIT ,Human GM2 ganglioside activator ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Ganglioside GM2 activator, GM2A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ganglioside GM2 activator. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ganglioside GM2 activator and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ganglioside GM2 activator, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ganglioside GM2 activator in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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