Human Keratinocyte differentiation- associated protein, KRTDAP E

Human Keratinocyte differentiation- associated protein, KRTDAP  ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

KRTDAP,KDAP; UNQ467; KIPV467; keratinocyte differentiation-associated protein

Search name

Human KRTDAP ELISA KIT ,Human KDAP ELISA KIT ,Human UNQ467 ELISA KIT ,Human KIPV467 ELISA KIT ,Human keratinocyte differentiation-associated protein ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Keratinocyte differentiation- associated protein, KRTDAP  concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to KRTDAP . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for KRTDAP  and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain KRTDAP , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of KRTDAP  in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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