Human DNA polymerase subunit gamma- 1, POLG ELISA KIT

Product Name:Human DNA polymerase subunit gamma- 1, POLG ELISA KIT
Packing:96T

Catalog No.:ELI-27686h

Gene Name:POLG

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:DNA polymerase subunit gamma-1(POLG

Alternative Name:POLG,DNA polymerase subunit gamma-1(POLG
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human DNA polymerase subunit gamma- 1, POLG ELISA KIT allows for the in vitro quantitative determination of DNA polymerase subunit gamma-1(POLG concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human DNA polymerase subunit gamma- 1, POLG ELISA KIT has been pre-coated with an DNA polymerase subunit gamma-1(POLG antibody specific to DNA polymerase subunit gamma-1(POLG .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for DNA polymerase subunit gamma-1(POLG and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DNA polymerase subunit gamma-1(POLG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of DNA polymerase subunit gamma-1(POLG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!