Human Protein FAN, FAN ELISA Kit

Human Protein FAN, FAN ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Nsmaf,FAN,Protein FAN,RAMD5; factor associated with N-SMase activation; factor associated with neutral sphingomyelinase activation; neutral sphingomyelinase (N-SMase) activation associated factor; neutral sphingomyelinase activation associated factor

Search name

Human Nsmaf ELISA KIT ,Human FAN ELISA KIT ,Human Protein FAN ELISA KIT ,Human RAMD5 ELISA KIT ,Human factor associated with N-SMase activation ELISA KIT ,Human factor associated with neutral sphingomyelinase activation ELISA KIT ,Human neutral sphingomyelinase (N-SMase) activation associated factor ELISA KIT ,Human neutral sphingomyelinase activation associated factor ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human protein fan,Protein FAN concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein FAN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Protein FAN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein FAN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Protein FAN in the samples is then determined by comparing the O.D. of the samples to the standard curve.