Human Angiopoietin- related protein 5, ANGPTL5 ELISA KIT

Human Angiopoietin-related protein 5, ANGPTL5 ELISA KIT

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Human ANGPTL5 ELISA KIT ,Human angiopoietin-like 5 ELISA KIT ,Human angiopoietin-like protein 5 ELISA KIT ,Human angiopoietin like 5 ELISA KIT ,Human ANGL5 ELISA KIT

Intended use

Human ANGPTL5 ELISA KIT allows for the in vitro quantitative determination of Human Angiopoietin-related protein 5 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Human ANGPTL5 ELISA KIT is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANGPTL5 , During the reaction, ANGPTL5 in the sample or standard competes with a fixed amount of biotin-labeled ANGPTL5  for sites on a pre-coated Monoclonal antibody specific to  ANGPTL5 . Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANGPTL5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.31-20ng/ml

Human ANGPTL5 ELISA KIT Manual download

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