Human A- kinase anchor protein 1, mitochondrial, AKAP1 ELISA KIT

Product Name:Human A- kinase anchor protein 1, mitochondrial, AKAP1 ELISA KIT
Packing:96T

Catalog No.:ELI-24227h

Gene Name:AKAP1

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:A-kinase anchor protein 1(mitochondrial(AKAP1

Alternative Name:AKAP1,A-kinase anchor protein 1(mitochondrial(AKAP1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human A- kinase anchor protein 1, mitochondrial, AKAP1 ELISA KIT allows for the in vitro quantitative determination of A-kinase anchor protein 1(mitochondrial(AKAP1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human A- kinase anchor protein 1, mitochondrial, AKAP1 ELISA KIT has been pre-coated with an A-kinase anchor protein 1(mitochondrial(AKAP1 antibody specific to A-kinase anchor protein 1(mitochondrial(AKAP1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for A-kinase anchor protein 1(mitochondrial(AKAP1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain A-kinase anchor protein 1(mitochondrial(AKAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of A-kinase anchor protein 1(mitochondrial(AKAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!