Human Multiple coagulation factor deficiency protein 2, MCFD2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Multiple coagulation factor deficiency protein 2,Neural stem cell-derived neuronal survival protein,MCFD2,F5F8D; F5F8D2; LMAN1IP; SDNSF; multiple coagulation factor deficiency protein 2; neural stem cell-derived neuronal survival protein; multiple coagulation factor deficiency 2
Search name
Human Multiple coagulation factor deficiency protein 2 ELISA KIT ,Human Neural stem cell-derived neuronal survival protein ELISA KIT ,Human MCFD2 ELISA KIT ,Human F5F8D ELISA KIT ,Human F5F8D2 ELISA KIT ,Human LMAN1IP ELISA KIT ,Human SDNSF ELISA KIT ,Human multiple coagulation factor deficiency protein 2 ELISA KIT ,Human neural stem cell-derived neuronal survival protein ELISA KIT ,Human multiple coagulation factor deficiency 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human multiple coagulation factor deficiency protein 2,MCFD2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MCFD2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MCFD2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MCFD2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MCFD2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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