Human Histidyl- tRNA synthetase, cytoplasmic, HARS ELISA KIT

Product Name:Human Histidyl- tRNA synthetase, cytoplasmic, HARS ELISA KIT
Packing:96T

Catalog No.:ELI-23603h

Gene Name:HARS

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Histidyl-tRNA synthetase(cytoplasmic(HARS

Alternative Name:HARS,Histidyl-tRNA synthetase(cytoplasmic(HARS
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Histidyl- tRNA synthetase, cytoplasmic, HARS ELISA KIT allows for the in vitro quantitative determination of Histidyl-tRNA synthetase(cytoplasmic(HARS concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Histidyl- tRNA synthetase, cytoplasmic, HARS ELISA KIT has been pre-coated with an Histidyl-tRNA synthetase(cytoplasmic(HARS antibody specific to Histidyl-tRNA synthetase(cytoplasmic(HARS .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Histidyl-tRNA synthetase(cytoplasmic(HARS and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Histidyl-tRNA synthetase(cytoplasmic(HARS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Histidyl-tRNA synthetase(cytoplasmic(HARS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!