Human ADP- ribose pyrophosphatase mitochondrial, NUDT9 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
NUDT9,NUDT10,PSEC0099,Nucleoside diphosphate-linked moiety X motif 9,Nudix motif 9;Adenosine diphosphoribose pyrophosphatase,ADPR-PPase;ADP-ribose diphosphatase;ADP-ribose phosphohydrolase,ADP-ribose pyrosphosphatase NUDT9; ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ADP-ribose pyrophosphatase mitochondrial; nudix (nucleoside diphosphate linked moiety X)-type motif 9
Search name
Human NUDT9 ELISA KIT ,Human NUDT10 ELISA KIT ,Human PSEC0099 ELISA KIT ,Human Nucleoside diphosphate-linked moiety X motif 9 ELISA KIT ,Human Nudix motif 9 ELISA KIT ,Human Adenosine diphosphoribose pyrophosphatase ELISA KIT ,Human ADPR-PPase ELISA KIT ,Human ADP-ribose diphosphatase ELISA KIT ,Human ADP-ribose phosphohydrolase ELISA KIT ,Human ADP-ribose pyrosphosphatase NUDT9 ELISA KIT ,Human ADP-ribose diphosphatase ELISA KIT ,Human ADP-ribose phosphohydrolase ELISA KIT ,Human ADP-ribose pyrophosphatase mitochondrial ELISA KIT ,Human nudix (nucleoside diphosphate linked moiety X)-type motif 9 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human ADP- ribose pyrophosphatase, mitochondrial, NUDT9 beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADP- ribose pyrophosphatase, mitochondrial, NUDT9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Human ADP- ribose pyrophosphatase, mitochondrial, NUDT9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human ADP- ribose pyrophosphatase, mitochondrial, NUDT9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human ADP- ribose pyrophosphatase, mitochondrial, NUDT9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.